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[1]鄢贵龙,纪丽莲,罗玉明,等.超声-微波协同提取野马追中抗氧化活性成分[J].南京工业大学学报(自然科学版),2013,35(01):1-71.[doi:10.3969/j.issn.1671-7627.2013.01.015]
 YAN Guilong,JI Lilian,LUO Yuming,et al.Ultrasonic-microwave synergistic extraction of antioxidants from Eupatorium lindleyanum DC.[J].Journal of NANJING TECH UNIVERSITY(NATURAL SCIENCE EDITION),2013,35(01):1-71.[doi:10.3969/j.issn.1671-7627.2013.01.015]
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超声-微波协同提取野马追中抗氧化活性成分()
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《南京工业大学学报(自然科学版)》[ISSN:1671-7627/CN:32-1670/N]

卷:
35
期数:
2013年01期
页码:
1-71
栏目:
出版日期:
2013-01-20

文章信息/Info

Title:
Ultrasonic-microwave synergistic extraction of antioxidants from Eupatorium lindleyanum DC.
文章编号:
1671-7627(2013)01-0071-05
作者:
鄢贵龙12纪丽莲2罗玉明2胡永红1
1. 南京工业大学 生物与制药工程学院,江苏 南京 210009; 2. 淮阴师范学院 江苏省环洪泽湖生态农业生物技术重点实验室,江苏 淮安 223300
Author(s):
YAN Guilong12 JI Lilian2 LUO Yuming2 HU Yonghong1
1. College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, China; 2. Jiangsu Key Laboratory of Eco-Agricultural Biotechnology around Hongze Lake, Huaiyin Normal University, Huaian 223300, China
关键词:
野马追 超声-微波协同作用 提取 抗氧化
Keywords:
Eupatorium lindleyanum DC. ultrasonic-microwave synergistic extraction extraction antioxidant activity
分类号:
R93;TS202.3
DOI:
10.3969/j.issn.1671-7627.2013.01.015
文献标志码:
A
摘要:
为了提高野马追提取物中抗氧化活性物质提取率,采用单因素和正交试验法,对超声-微波协同萃取野马追中抗氧化活性成分提取工艺条件进行了优化。结果表明:其最佳工艺参数为超声开启时,以体积分数20%的乙醇为溶剂,提取时间30 min,料液比(g/mL)为1:30,微波功率为50 W。在优化条件下提取获得的野马追提取物对1,1-二苯基-2-三硝基苯肼(DPPH·)清除作用IC50值为73.9 mg/mL,其总酚含量与抗氧化活性之间存在很强的相关性(R2=0.952 3)。与其他方法相比,超声-微波协同萃取法具有节省时间、节约能量、抗氧化活性成分收率高等优点。
Abstract:
To increase the yield rate of antioxidants from Eupatorium lindleyanum DC., ultrasonic-microwave synergistic extraction technique was applied to extracting antioxidants. The effects of main factors on extraction were discussed based on the single factor test and the orthogonal experiment. Results showed that the optimum extraction conditions were as follows: the extraction solvent was 20% ethanol solution, the extraction time was 30 min, the solid-liquid ratio was 1:30, the microwave power was 50 W, and the ultrasonic was turned on. Under the optimized conditions, the IC50 value of the extraction was 73.9 mg/L, and the content of total phenolics showed a good correlation with DPPH· radical scavenging activity(R2=0.952 3). Compared with other extraction methods, the ultrasonic-microwave synergistic extraction was a better method for antioxidant extraction from Eupatorium lindleyanum DC. with less time and higher recovery rate of antioxidants.

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备注/Memo

备注/Memo:
收稿日期:2012-05-09
基金项目:江苏省研究生科研创新计划(CX10B_182Z); 江苏省生物质能与酶技术重点实验室开发课题(JSBEET1222)
作者简介:鄢贵龙(1975—),男,江西临川人,博士生,主要研究方向为发酵工程、天然产物研究与开发; 胡永红(联系人),教授,E-mail:hyh@njut.edu.cn..
更新日期/Last Update: 2013-01-20