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[1]王学超,李延报,陆春华,等.水溶性丝素蛋白的制备及其构象转变[J].南京工业大学学报(自然科学版),2013,35(02):55-58.[doi:10.3969/j.issn.1671-7627.2013.02.011]
 WANG Xuechao,LI Yanbao,LU Chunhua,et al.Preparation and conformation transformation of water-soluble silk fibroin[J].Journal of NANJING TECH UNIVERSITY(NATURAL SCIENCE EDITION),2013,35(02):55-58.[doi:10.3969/j.issn.1671-7627.2013.02.011]
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水溶性丝素蛋白的制备及其构象转变()
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《南京工业大学学报(自然科学版)》[ISSN:1671-7627/CN:32-1670/N]

卷:
35
期数:
2013年02期
页码:
55-58
栏目:
出版日期:
2013-03-20

文章信息/Info

Title:
Preparation and conformation transformation of water-soluble silk fibroin
文章编号:
1671-7627(2013)02-0055-04
作者:
王学超李延报陆春华许仲梓
南京工业大学 材料科学与工程学院,江苏 南京 210009
Author(s):
WANG XuechaoLI YanbaoLU ChunhuaXU Zhongzi
College of Materials Science and Engineering,Nanjing University of Technology,Nanjing 210009,China
关键词:
水溶性 丝素蛋白 构象转变
Keywords:
water-solubility silk fibroin conformation transformation
分类号:
O629.73;O631.1
DOI:
10.3969/j.issn.1671-7627.2013.02.011
文献标志码:
A
摘要:
通过液氮淬冷和冷冻干燥的方法获得了粒度均一、直径为3~4 mm的水溶性丝素蛋白微珠,并将丝素蛋白微珠分别进行室温保存、-20 ℃下保存和乙醇浸泡等不同方法处理。通过扫描电子显微镜(SEM)、傅里叶变换红外光谱仪(FT-IR)和X线衍射仪(XRD)等分析技术表征丝素蛋白微珠的形貌、构象和晶相组成。结果表明:液氮淬冷和冷冻干燥制备的丝素蛋白主要为无规线团结构,易溶于水; 在室温和-20 ℃下放置1周后的丝素蛋白发生了由无规线团向β-转角、α-螺旋和β-折叠等构象转变,水溶性变差; 经乙醇处理后的丝素蛋白因含有大量的β-折叠构象则不溶于水。
Abstract:
The water-soluble silk fibroin(SF)beads with a uniform particle size and a diameter range of 3-4 mm were prepared by the liquid nitrogen quenching and freeze-drying process,then kept at room temperature,-20 ℃ preservation,and treated by soaking in alcohol for 30 min.The morphologies,conformations and crystal phases of SF beads were characterized by scanning electron microscopy(SEM),Fourier transform infrared spectroscopy(FT-IR)and X-ray diffraction(XRD).It was found that the conformation of the as-prepared silk protein was almost random coils,so it was soluble in water.The fibroin conformation changed into β-turn,α-helix and β-sheet structures after the storage for one week at room temperature or -20 ℃,while the water solubility of the SF beads was gradually impaired.And the silk protein was insoluble in water after the treatment with ethanol for a good number of β-sheet.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2012-03-29
基金项目:国家自然科学基金(50802042); 江苏省自然科学基金(BK2011076)
作者简介:王学超(1985—),男,安徽芜湖人,硕士,主要研究方向为生物医用材料; 李延报(联系人),副教授,E-mail:ybli@njut.edu.cn..
更新日期/Last Update: 2013-03-31