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[1]汤俊,郝宁,许晟,等.谷氨酸棒杆菌argG基因缺失菌株的构建[J].南京工业大学学报(自然科学版),2013,35(06):86-90.[doi:10.3969/j.issn.1671-7627.2013.06.018]
 TANG Jun,HAO Ning,XU Sheng,et al.Construction of Corynebacterium glutamicum mutant with knockout of argG gene[J].Journal of NANJING TECH UNIVERSITY(NATURAL SCIENCE EDITION),2013,35(06):86-90.[doi:10.3969/j.issn.1671-7627.2013.06.018]
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谷氨酸棒杆菌argG基因缺失菌株的构建()
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《南京工业大学学报(自然科学版)》[ISSN:1671-7627/CN:32-1670/N]

卷:
35
期数:
2013年06期
页码:
86-90
栏目:
出版日期:
2013-11-20

文章信息/Info

Title:
Construction of Corynebacterium glutamicum mutant with knockout of argG gene
文章编号:
1671-7627(2013)06-0086-05
作者:
汤俊郝宁许晟许琳严明
南京工业大学 生物与制药工程学院,江苏 南京 210009
Author(s):
TANG JunHAO NingXU ShengXU LinYAN Ming
College of Biotechnology and Pharmaceutical Enginneering,Nanjing University of Technology,Nanjing 210009,China
关键词:
谷氨酸棒杆菌 L-瓜氨酸 基因敲除
Keywords:
Corynebacterium glutamicum L-citrulline gene knockout
分类号:
TQ922+.9
DOI:
10.3969/j.issn.1671-7627.2013.06.018
文献标志码:
A
摘要:
构建1株谷氨酸棒杆菌argG基因缺失菌株并观察其对L-瓜氨酸生产的影响。采用crossover PCR方法扩增带有argG基因上下游同源序列的DNA片段,连接至载体pK18mobsacB,构建敲除载体pK18-ΔargG,转化至C. glutamicum ATCC 13032,利用卡那霉素抗性正筛选和果聚糖蔗糖sacB基因负筛选获得argG基因缺失菌株。发酵结果表明,argG缺失菌株60 h积累2.52 g/L L-瓜氨酸,出发菌C. glutamicum ATCC 13032不能积累L-瓜氨酸。
Abstract:
Construction of argG-deleted mutant and effect of argG inactivation on L-citrulline production in Corynebacterium glutamicum were investigated. For deletion of argG gene in C.glutamicum ATCC 13032,crossover PCR was carried out to generate the argG deletion fragment ligated to the suicide vector pK18mobsacB. The recombinant plasmid pK18-ΔargG was transferred into C. glutamicum by electroporation. C. glutamicum ATCC 3032-ΔargG was successfully obtained by double homologous recombination. The fermentation result showed that L-citrulline production of the argG gene knockout strain was achieved up to 2.52 g/L.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2013-03-28
基金项目:江苏省普通高校研究生科研创新计划(CXLX11_0373)
作者简介:汤俊(1987—),男,江苏南通人,硕士,主要研究方向为微生物基因工程与代谢工程; 郝宁(联系人),副教授,E-mail:haoning@njut.edu.cn..
更新日期/Last Update: 2013-11-30