|本期目录/Table of Contents|

[1]段许佳,卢圣国,罗芳,等.琼脂糖-玻璃珠亲和色谱填料合成及分离应用[J].南京工业大学学报(自然科学版),2014,36(03):82-88.[doi:10.3969/j.issn.1671-7627.2014.03.016]
 DUAN Xujia,LU Shengguo,LUO Fang,et al.Preparation of agarose-glass composite particles for affinity purification of glycoproteins[J].Journal of NANJING TECH UNIVERSITY(NATURAL SCIENCE EDITION),2014,36(03):82-88.[doi:10.3969/j.issn.1671-7627.2014.03.016]
点击复制

琼脂糖-玻璃珠亲和色谱填料合成及分离应用()
分享到:

《南京工业大学学报(自然科学版)》[ISSN:1671-7627/CN:32-1670/N]

卷:
36
期数:
2014年03期
页码:
82-88
栏目:
出版日期:
2014-05-20

文章信息/Info

Title:
Preparation of agarose-glass composite particles for affinity purification of glycoproteins
文章编号:
1671-7627(2014)03-0082-07
作者:
段许佳1卢圣国1罗芳1黄和1韩毓旺2
1.南京工业大学 生物与制药工程学院,江苏 南京 211800; 2.南京工业大学 理学院,江苏 南京 211800
Author(s):
DUAN Xujia1LU Shengguo1LUO Fang1HUANG He1HAN Yuwang2
1.College of Life Science and Pharmacy,Nanjing Tech University,Nanjing 211800,China; 2.College of Sciences,Nanjing Tech University,Nanjing 211800,China
关键词:
油水乳化 复合材料 琼脂糖 氨基苯硼酸 糖蛋白
Keywords:
water-in-oil emulsification composite particles agarose m-aminophenyboronic acid(APBA) glycoprotein
分类号:
O657
DOI:
10.3969/j.issn.1671-7627.2014.03.016
文献标志码:
A
摘要:
利用油水乳化法,制备具有壳-核结构的琼脂糖/玻璃珠复合材料,并偶联氨基苯硼酸,得到一种新型的亲和填料。对影响制备过程的琼脂糖/玻璃珠质量比、乳化剂的含量、环氧基团活化密度进行了优化。采用光学显微镜、激光粒度分析仪、红外光谱仪、热质量分析仪等对这种复合介质进行了表征,并考察亲和介质的吸附性能。结果表明:复合材料对辣辣根过氧化物酶(糖蛋白)的饱和吸附量为85 mg/g,远大于牛血清蛋白(非糖蛋白)6 mg/g 的吸附量,并在2 mL/min 流速下实现了对人血红细胞中糖化血红蛋白与非糖化血红蛋白的分离,在亲和色谱方面具有较大的应用前景。
Abstract:
M-aminophenyboronic acid(APBA)-functionalized agarose-glass composite particles were fabricated by water-in-oil emulsification for a specific affinity to glycoproteins.The reaction conditions were optimized,including the ratio of agarose to glass,the content of emulsifier and epichlorohydrin(ECH).The affinity particles were characterized by optical microscope,laser particle size analyzer,Fourier transform infrared spectroscopy(FT-IR), and thermogravimetry(TG).The saturated adsorption capacity of horse radish peroxidase(HRP)was 85 mg/g,which was remarkably higher than that obtained for bovine serum albumin(BSA).It could be applied to the specific capture of the glycosylated hemoglobin at 2 mL/min in chromatography with a potential application in affinity chromatography.

参考文献/References:

[1] Tiainen P,Gustavsson P E,Ljunglöf A,et al.Superporous agarose anion exchangers for plasmid isolation[J].Journal of Chromatography A,2007,1138(1/2):84-94.
[2] Larsen M R,ThingholmT E,Jensen O N,et al.Highly selective enrichment of phosphorylated peptides from peptide mixtures using titanium dioxide microcolumns[J].Molecular & Cellular Proteomics,2005,4(7):873-886.
[3] Kweon H K,Hakansson K.Selective zirconium dioxide-based enrichment of phosphorylated peptides for mass spectrometric analysis[J].Analytical chemistry,2006,78(6):1743-1749.
[4] Buchmeiser M R.New synthetic ways for the preparation of high-performance liquid chromatography supports[J].Journal of Chromatography A,2001,918(2):233-266.
[5] Zhou X,Sun Y,Liu Z.Superporous pellicular agarose-glass composite particle for protein adsorption[J].Biochemical Engineering Journal,2007,34(2):99-106.
[6] Jin G,Yao Q Z,Zhang L.Adsorption of human serum albumin onto PVA-coated affinity microporous PTFE capillary[J].Chemical Research in Chinese Universities,2008,24(2):154-161.
[7] Xu X,Dong P,Feng Y,et al.A simple strategy for preparation of spherical silica-supported porous chitosan matrix based on sol-gel reaction and simple treatment with ammonia solution[J].Analytical Methods,2010,2(5):546-551.
[8] Miao Z J,Lin D Q,Yao S J.Preparation and characterization of cellulose-stainless steel powder composite particles customized for expanded bed application[J].Industrial & Engineering Chemistry Research,2005,44(22):8218-8224.
[9] Marth J D,Grewal P K.Mammalian glycosylation in immunity[J].Nature Reviews Immunology,2008,8(11):874-887.
[10] Du K F,Yang D,Sun Y.Controlled fabrication of porous titania beads by a sol-gel templating method[J].Industrial & Engineering Chemistry Research,2009,48(2):755-762.
[11] Scoble J A,Scopes R K.Assay for determining the number of reactive groups on gels used in affinity chromatography and its application to the optimisation of the epichlorohydrin and divinylsulfone activation reactions[J].Journal of Chromatography A,1996,752(1/2):67-76.
[12] 张松平,丁锐,苏志国,等.无水体系中环氧氯丙烷活化琼脂糖凝胶制备高载量固定化金属亲和层析介质[J].过程工程学报,2010,10(5):971-975.
[13] Zhang H Y,Yao G P,Deng C H,et al.Facile synthesis of boronic acid-functionalized magnetic mesoporous silica nanocomposites for highly specific enrichment of glycopeptides[J].Chinese Journal of Chemistry,2011,29(4):835-839.
[14] Bisse E,Wieland H.Coupling of m-aminophenylboronic acid to s-triazine-activated sephacryl:use in the affinity chromatography of glycated hemoglobins[J].Journal of Chromatography B:Biomedical Sciences and Applications,1992,575(2):223-228.

相似文献/References:

[1]吴战武,刘渝,沈晓冬,等.MTES疏水改性SiO2气凝胶修饰活性炭复合材料的制备及结构表征[J].南京工业大学学报(自然科学版),2012,34(03):103.[doi:10.3969/j.issn.1671-7627.2012.03.021]
 WU Zhanwu,LIU Yu,SHEN Xiaodong,et al.Preparation and characterization of activated carbon composite materials modified by MTES hydrophobic silica aerogel[J].Journal of NANJING TECH UNIVERSITY(NATURAL SCIENCE EDITION),2012,34(03):103.[doi:10.3969/j.issn.1671-7627.2012.03.021]
[2]李乔钧,陆雪非,尤加健,等.MPPO/talc复合材料制备及其性能研究[J].南京工业大学学报(自然科学版),1996,18(01):40.
 Li Qiaojun,Lu Xuefei,You Jiajian,et al.STUDY ON PREPARATION OF MPPO/TALC COMPOSITE AND ITS PROPERTIES[J].Journal of NANJING TECH UNIVERSITY(NATURAL SCIENCE EDITION),1996,18(03):40.
[3]马功勋.各向异性板弯曲的数值解[J].南京工业大学学报(自然科学版),1996,18(01):58.
 Ma Gongxun (Department of Mechanical Engineering,Nanjing University of Chemical Technology,Nanjing,et al.NUMERICAL SOLUTION FOR BENDING OF ANISOTROPY PLATES[J].Journal of NANJING TECH UNIVERSITY(NATURAL SCIENCE EDITION),1996,18(03):58.
[4]王志远,沈旭,俞娟,等.纳米炭黑/聚酰亚胺复合材料的摩擦磨损性能[J].南京工业大学学报(自然科学版),2015,37(02):28.[doi:10.3969/j.issn.1671-7627.2015.02.006]
 WANG Zhiyuan,SHEN Xu,YU Juan,et al.Friction and wear performance of nanometer carbon black/polyimide composite material[J].Journal of NANJING TECH UNIVERSITY(NATURAL SCIENCE EDITION),2015,37(03):28.[doi:10.3969/j.issn.1671-7627.2015.02.006]
[5]张斌,顾伯勤,宇晓明.大模量比短纤维增强复合材料应力分布预测[J].南京工业大学学报(自然科学版),2015,37(02):65.[doi:10.3969/j.issn.1671-7627.2015.02.013]
 ZHANG Bin,GU Boqin,YU Xiaoming.Prediction of stress distribution in short-fiber-reinforced composites of large modulus ratio[J].Journal of NANJING TECH UNIVERSITY(NATURAL SCIENCE EDITION),2015,37(03):65.[doi:10.3969/j.issn.1671-7627.2015.02.013]
[6]宇晓明,顾伯勤,张斌,等.短纤维端部形状对其增强复合材料应力分布的影响[J].南京工业大学学报(自然科学版),2015,37(02):75.[doi:10.3969/j.issn.1671-7627.2015.02.015]
 YU Xiaoming,GU Boqin,ZHANG Bin,et al.Effects of fiber tip geometry on stress distribution of short fiber reinforced composites[J].Journal of NANJING TECH UNIVERSITY(NATURAL SCIENCE EDITION),2015,37(03):75.[doi:10.3969/j.issn.1671-7627.2015.02.015]
[7]曾少华,申明霞,李佳骐,等.MWCNT对玻纤复合材料界面黏合性及其影响机制[J].南京工业大学学报(自然科学版),2017,39(05):27.[doi:10.3969/j.issn.1671-7627.2017.05.005]
 ZENG Shaohua,SHEN Mingxia,LI Jiaqi,et al.Effects of MWCNT on interfacial adhesion of glass fiber composites and its reinforcing mechanisms[J].Journal of NANJING TECH UNIVERSITY(NATURAL SCIENCE EDITION),2017,39(03):27.[doi:10.3969/j.issn.1671-7627.2017.05.005]
[8]邓国良,邓建新,黄家成,等.南昌大桥桥墩抗浮运管段撞击能力评估及复合材料防撞设计[J].南京工业大学学报(自然科学版),2017,39(05):115.[doi:10.3969/j.issn.1671-7627.2017.05.018]
 DENG Guoliang,DENG Jianxin,HUANG Jiacheng,et al.Assessment of resistance to floating pipe impact capability and protection method of Nanchang Bridge piers[J].Journal of NANJING TECH UNIVERSITY(NATURAL SCIENCE EDITION),2017,39(03):115.[doi:10.3969/j.issn.1671-7627.2017.05.018]

备注/Memo

备注/Memo:
收稿日期:2013-09-26
作者简介:段许佳(1987—),男,天津人,硕士生,主要研究方向色谱填料的制备; 韩毓旺(联系人),教授,E-mail: hanyw@njtech.edu.cn..
更新日期/Last Update: 2014-05-20