|本期目录/Table of Contents|

[1]满宁,孙盼,韩伟.灰树花子实体多糖的微波提取及絮凝纯化工艺[J].南京工业大学学报(自然科学版),2015,37(06):99-104.[doi:10.3969/j.issn.1671-7627.2015.06.018]
 MAN Ning,SUN Pan,HAN Wei.Application of microwave-flocculation technology in extraction and purification of polysaccharides from Grifola frondosa[J].Journal of NANJING TECH UNIVERSITY(NATURAL SCIENCE EDITION),2015,37(06):99-104.[doi:10.3969/j.issn.1671-7627.2015.06.018]
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灰树花子实体多糖的微波提取及絮凝纯化工艺()
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《南京工业大学学报(自然科学版)》[ISSN:1671-7627/CN:32-1670/N]

卷:
37
期数:
2015年06期
页码:
99-104
栏目:
出版日期:
2015-11-20

文章信息/Info

Title:
Application of microwave-flocculation technology in extraction and purification of polysaccharides from Grifola frondosa
文章编号:
1671-7627(2015)06-0099-06
作者:
满宁孙盼韩伟
华东理工大学 药学院 中药现代化工程中心,上海 200237
Author(s):
MAN NingSUN PanHAN Wei
Engineering Center for Traditional Chinese Medicine Modernization,School of Pharmacy, East China University of Science and Technology,Shanghai 200237,China
关键词:
灰树花 多糖 微波 絮凝
Keywords:
Grifola frondosa polysaccharides microwave flocculation
分类号:
R284.2
DOI:
10.3969/j.issn.1671-7627.2015.06.018
文献标志码:
A
摘要:
以得率为指标,去离子水为溶剂,微波提取灰树花多糖,得到最佳提取工艺为:药材粒径840~2 000 μm,液固比30 mL/g,微波提取210 s,溶剂pH=6; 以脱蛋白率和多糖保留率为指标,壳聚糖为絮凝剂纯化灰树花多糖提取液,得到最佳工艺参数为:壳聚糖1.0 mL/g,絮凝1 h,液药比40 mL/g,药液pH=6,温度控制在45 ℃。与其他工艺相比,本文采用的微波提取及絮凝纯化方法操作高效,能有效提高灰树花多糖的得率和纯度。
Abstract:
Polysaccharides in Grifola frondosa were extracted by deionized water with microwave.Extraction yield as the index,optimum conditions were as follows:herbs diameter of 840-2 000 μm,liquid-solid ratio of 30 mL/g,extraction time of 210 s,and solvent pH of 6.Polysaccharides were purified using chitosan as flocculant.With removal rate of protein and retention rate of polysaccharides as indexes,optimal parameters of flocculation purification were as follows:coagulant dosage of 1.0 mL/g,flocculation time of 1 h,liquid-drug ratio of 40 mL/g,drug solution pH of 6,and flocculation-temperature of 45 ℃.Compared with other operations,the method for microwave extraction and flocculation purification had the advantages of high operating efficiency,improving extraction yield and purity of polysaccharides.

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备注/Memo

备注/Memo:
收稿日期:2014-07-14
基金项目:上海市科技兴农重点攻关项目[沪农科攻字(2012)第2-9号]
作者简介:满宁(1989—),女,辽宁本溪人,硕士,主要研究方向为天然产物的分离与纯化; 韩伟(联系人),教授,E-mail:whan@ecust.edu.cn.
引用本文:满宁,孙盼,韩伟.灰树花子实体多糖的微波提取及絮凝纯化工艺[J].南京工业大学学报:自然科学版,2015,37(6):99-104..
更新日期/Last Update: 2015-11-20