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[1]崔广波,刘晓昕.基于气相色谱-质谱的代谢组学技术研究紫杉醇诱导的卵巢癌A2780耐药株的生物标志物[J].南京工业大学学报(自然科学版),2016,38(01):111-116.[doi:10.3969/j.issn.1671-7627.2016.01.018]
 CUI Guangbo,LIU Xiaoxin.Metabolomic profiling based on gas chromatography-mass spectrometer(GC-MS)for identification of potential biomarkers in paclitaxel-resistant A2780 ovarian cancer cell[J].Journal of NANJING TECH UNIVERSITY(NATURAL SCIENCE EDITION),2016,38(01):111-116.[doi:10.3969/j.issn.1671-7627.2016.01.018]
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基于气相色谱-质谱的代谢组学技术研究紫杉醇诱导的卵巢癌A2780耐药株的生物标志物()
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《南京工业大学学报(自然科学版)》[ISSN:1671-7627/CN:32-1670/N]

卷:
38
期数:
2016年01期
页码:
111-116
栏目:
出版日期:
2016-01-10

文章信息/Info

Title:
Metabolomic profiling based on gas chromatography-mass spectrometer(GC-MS)for identification of potential biomarkers in paclitaxel-resistant A2780 ovarian cancer cell
文章编号:
1671-7627(2016)01-0111-06
作者:
崔广波刘晓昕
南京工业大学 药学院,江苏 南京 210009
Author(s):
CUI GuangboLIU Xiaoxin
College of Pharmaceutical Science,Nanjing Tech University,Nanjing 210009,China
关键词:
卵巢癌 紫杉醇 耐药蛋白 代谢组学
Keywords:
ovarian cancer taxol resistance protein metabolomic
分类号:
R979.1
DOI:
10.3969/j.issn.1671-7627.2016.01.018
文献标志码:
A
摘要:
采用气相色谱-质谱(GC-MS)的代谢组学技术研究人卵巢癌紫杉醇耐药细胞A2780/Taxol与敏感细胞之间内源性代谢物的差异,初步探讨A2780/Taxol细胞生物学特性和耐药机制。使用浓度梯度法建立A2780/Taxol细胞,并通过细胞形态观察,生长曲线测定,药物敏感试验,细胞内多药耐药相关蛋白(MRP1)、P-糖蛋白(P-gp)和肺耐药蛋白(LRP)的水平测定,评价A2780/Taxol细胞的生物学特性。同时,采用GC-MS的代谢组学方法分别对卵巢癌敏感细胞株与紫杉醇耐药细胞株的代谢物指纹图谱进行分析,并通过偏最小二乘法判别分析(PLS-DA)对代谢组学数据进行多元处理。结果表明:人卵巢癌细胞敏感细胞与耐药细胞相比,其中琥珀酸、天冬氨酸、果糖、肌醇等13种内源性代谢物存在显著性差异。糖降解、三羧酸(TCA)循环、肌苷代谢途径发生了异常改变,这些差异改变可能与卵巢癌细胞的耐药机制有关。
Abstract:
Gas chromatography-mass spectrometer(GC-MS)metabolomic was used to identify the differences of potential metabolic between human ovarian cancer paclitaxel-resistant cells(A2780/Taxol)and sensitive ones.Meanwhile,it was used to research biological characteristics and resistance mechanisms of paclitaxel-resistant cells.Paclitaxel-resistant cells were induced by continuous stepwise selection with increasing concentrations of taxol.The biological characteristics were identified by evaluating cell morphology,growth curve,drug susceptibility testing, and intracellular protein expression measurement of multidrug-associated protein(MRP1), P-glycoprotein(P-gp)and lung resistance protein(LRP).GC-MS metabolomics was used to investigate endogenous metabolic changes in A2780 paclitaxel-resistant cells.The metabolic differences of paclitaxel-resistant cells were compared by sensitive cells using partial least squares-discriminant analysis(PLS-DA)score plot analysis.Results showed that significant metabolite differences were also observed between human ovarian cancer paclitaxel-resistant cells and sensitive ones.13 characteristic metabolites, such as succinic acid,aspartic acid,fructose,and inositol et al were identified.Abnormal alterations in metabolic pathways were identified,namely,the glycolysis, tricarboxylic acid(TCA)cycle,inosine metabolism,which may be involved in resistance mechanisms of ovarian cancer cells.

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备注/Memo

备注/Memo:
收稿日期:2014-12-13
作者简介:崔广波(1988—),男,山东青岛人,硕士,主要研究方向为药理学; 刘晓昕(联系人),实习研究员,博士生,E-mail:liuxiaoxin@ njtech.edu.cn.
引用本文:崔广波,刘晓昕.基于气相色谱-质谱的代谢组学技术研究紫杉醇诱导的卵巢癌A2780耐药株的生物标志物[J].南京工业大学学报(自然科学版),2016,38(1):111-116..
更新日期/Last Update: 2016-01-20