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[1]杨晓瑞,任丽滨,王倩倩,等.光合细菌固定CO2偶联净化沼液[J].南京工业大学学报(自然科学版),2016,38(03):114-119.[doi:10.3969/j.issn.1671-7627.2016.03.019]
　YANG Xiaorui,REN Libin,WANG Qianqian,et al.Carbon dioxide fixing with simultaneous biogas slurry purification by photosynthetic bacteria[J].Journal of NANJING TECH UNIVERSITY(NATURAL SCIENCE EDITION),2016,38(03):114-119.[doi:10.3969/j.issn.1671-7627.2016.03.019]
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光合细菌固定CO2偶联净化沼液()
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《南京工业大学学报（自然科学版）》[ISSN:1671-7627/CN:32-1670/N]

卷:
38
期数:
2016年03期
页码:
114-119
栏目:
出版日期:
2016-05-28

文章信息/Info

Title:
Carbon dioxide fixing with simultaneous biogas slurry purification by photosynthetic bacteria
文章编号:
1671-7627(2016)03-0114-06
作者:
杨晓瑞1; 任丽滨2; 王倩倩1; 刘超英1; 梁金花1; 朱建良1
1.南京工业大学 生物与制药工程学院,江苏 南京 211800; 2.聊城职业技术学院,山东 聊城 252000
Author(s):
YANG Xiaorui1; REN Libin2; WANG Qianqian1; LIU Chaoying1; LIANG Jinhua1; ZHU Jianliang1
1. College of Biotechnology and Pharmaceutical Engineering,Nanjing Tech University,Nanjing 211800,China; 2. Liaocheng Vocational and Technical College,Liaocheng 252000,China
关键词:
光合细菌;  CO2固定速率;  沼液COD去除率
Keywords:
photosynthetic bacteria;  carbon dioxide fixation rate;  biogas slurry COD removal rate
分类号:
X505
DOI:
10.3969/j.issn.1671-7627.2016.03.019
文献标志码:
A
摘要:
针对大型沼气工程CO2含量高、沼气利用率低及沼液中有机物含量高等问题,筛选得到能同时固定CO2和去除沼液中有机物的光合细菌沼泽红假单胞菌(NJ),并对其进行条件试验。以模拟沼液固定化培养基为底物,考察光照强度、菌种的接种量和菌龄、培养基中Na2S的添加量等因素对固定CO2速率的影响。以沼液为底物,考察沼液的稀释倍数和电子供体对光合细菌生长、固定CO2速率和沼液化学需氧量(COD)去除率的影响。结果表明:以模拟沼液为培养基时,在温度35 ℃、光照强度1 500 lx、接种量0.1 g/L、菌龄60 h及培养基中Na2S 添加量2.0 g/L条件下,固定CO2速率达205 mL/h(以1 g干细胞计)。初始沼液COD 3 g/L,将沼液稀释10倍,蔗糖添加量为0.15 g/L,MgCL2添加量为0.06 g/L的条件下,光合细菌NJ生长最快,光密度(OD)值由0.24增大到0.48; 固定CO2速率达到最大,为218 mL/h。此时,沼液COD去除率为93%。
Abstract:
Screened photosynthetic bacteria Rhodopseudoumonas palustris(NJ)was used for fixing carbon dioxide and removing organic matter in biogas slurry and the condition test was studied to solve problems in large-scale biogas project,such as high CO2 content,low biogas utilization,high organic content were studied. Immobilized substrate was used as analog biogas slurry to investigate the factors effecting CO2 fixation rate such as the light intensity,bacteria inoculation amount and ages,medium addition of Na2S. Biogas dilution factor and the added electron donor for CO2 fixation rate and slurry COD removal rate were further investigated using biogas slurry as substrate. Results showed as follows:when analog biogas slurry as substrate,at temperature of 35 ℃,light intensity of 1 500 lx,inoculum amount of 0.1 g/L, bateria age of 60 h and Na2S amount of 2.0 g/L,CO2 fixed rate was up to 205 mL/h(in 1 g dry cell). Under conditions of initial biogas slurry COD of 3 g/L,dilution of 10 times,sucrose of 0.15 g/L and MgCl2 of 0.06 g/L,NJ could obtain the fastest growing speed, optical density(OD)value increased from 0.24 to 0.48,and CO2 fixation rate reached the maximum of 218 mL/h. Meanwhile, biogas slurry COD removal rate was 93%.

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备注/Memo

备注/Memo:
收稿日期:2015-01-07
基金项目:国家高技术研究发展计划(“863”计划重大项目)(2011AA02203)
作者简介:杨晓瑞(1983—),女,山西太谷人,博士生,主要研究方向为可再生能源; 朱建良(联系人),教授,E-mail:jlzhu@njtech.edu.cn.
引用本文:杨晓瑞,任丽滨,王倩倩,等.光合细菌固定CO2偶联净化沼液[J].南京工业大学学报(自然科学版),2016,38(3):114-119..
更新日期/Last Update: 2016-05-20