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[1]仲月,朱瑾,冯慧,等.定量检测血清降钙素原免疫层析试纸条的研制[J].南京工业大学学报(自然科学版),2016,38(04):125-129.[doi:10.3969/j.issn.1671-7627.2016.04.021]
 ZHONG Yue,ZHU Jin,FENG Hui,et al.Development of immunochromatography test strips for quantitative procalcitonin detection in serum[J].Journal of NANJING TECH UNIVERSITY(NATURAL SCIENCE EDITION),2016,38(04):125-129.[doi:10.3969/j.issn.1671-7627.2016.04.021]
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定量检测血清降钙素原免疫层析试纸条的研制()
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《南京工业大学学报(自然科学版)》[ISSN:1671-7627/CN:32-1670/N]

卷:
38
期数:
2016年04期
页码:
125-129
栏目:
出版日期:
2016-07-10

文章信息/Info

Title:
Development of immunochromatography test strips for quantitative procalcitonin detection in serum
文章编号:
1671-7627(2016)04-0125-05
作者:
仲月朱瑾冯慧韩毓旺张红漫
南京工业大学 化学与分子工程学院,江苏 南京 211800
Author(s):
ZHONG YueZHU JinFENG HuiHAN YuwangZHANG Hongman
College of Chemistry and Molecular Engineering,Nanjing Tech University,Nanjing 211800,China
关键词:
降钙素原 免疫层析 荧光胶乳
Keywords:
procalcitonin immunochromatography fluorescent latex
分类号:
R446.6
DOI:
10.3969/j.issn.1671-7627.2016.04.021
文献标志码:
A
摘要:
将荧光胶乳微球用1株降钙素原(PCT)单抗进行标记,采用双抗体夹心法,制备定量检测降钙素原免疫层析试纸条。与罗氏电化学发光法对比,138 例血清测试的阴、阳性符合率分别为96.7%和97.2%,质量浓度为0.1~100 ng/mL时,2种方法有显著相关性,在0.5~10 ng/mL时,差异无统计学意义。该试纸条检测限为0.09 ng/mL,在4 ℃至少可保存12个月,稳定性良好。建立的检测PCT的方法具有快速、灵敏、稳定等特点,可用于临床检测和预后。
Abstract:
Based on the double antibody sandwich technique,immunochromatography test strips for quantitative procalcitonin(PCT)detection was developed by labeling a mono antibody on fluorescence latex microspheres. The measurement of 138 serum samples revealed that the negative and positive coincidence rate were 96.7% and 97.2%, respectively,compared with the results obtained from Roche’s electrochemiluminesence assay. With concentration from 0.1 to 100 ng/mL,the two methods had good correlation,without statistically significant at 0.5-10 ng/mL. The detection limit was 0.09 ng/mL and the test strips were steable for more than one year when preserved at 4 ℃ under dry conditions. So it was rapid and sensitive for measuring PCT with long-term stablity,and could be used for diagnosis and prognosis.

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备注/Memo

备注/Memo:
收稿日期:2015-03-10
作者简介:仲月(1990—),女,江苏宿迁人,硕士,主要研究方向为体外诊断试剂; 韩毓旺(联系人),教授,E-mail:hanyw@njtech.edu.cn.
引用本文:仲月,朱瑾,冯慧,等.定量检测血清降钙素原免疫层析试纸条的研制[J].南京工业大学学报(自然科学版),2016,38(4):125-129..
更新日期/Last Update: 2016-07-10