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[1]武童,汪振炯,华春,等.基于大肠杆菌MG1655载体矿化合成纳米硒的研究[J].南京工业大学学报(自然科学版),2017,39(03):52-57.[doi:10.3969/j.issn.1671-7627.2017.03.010]
 WU Tong,WANG Zhenjiong,HUA Chun,et al.Biomineral synthesis of nano-selenium with Escherichia coli MG1655 as intermediary[J].Journal of NANJING TECH UNIVERSITY(NATURAL SCIENCE EDITION),2017,39(03):52-57.[doi:10.3969/j.issn.1671-7627.2017.03.010]
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基于大肠杆菌MG1655载体矿化合成纳米硒的研究()
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《南京工业大学学报(自然科学版)》[ISSN:1671-7627/CN:32-1670/N]

卷:
39
期数:
2017年03期
页码:
52-57
栏目:
出版日期:
2017-05-20

文章信息/Info

Title:
Biomineral synthesis of nano-selenium with Escherichia coli MG1655 as intermediary
文章编号:
1671-7627(2017)03-0052-06
作者:
武童12汪振炯2华春2周峰1王仁雷3黄和1
1. 南京工业大学 生物与制药工程学院,江苏 南京 211800; 2. 南京晓庄学院 食品科学学院,江苏 南京 211171; 3. 江苏第二师范学院 生物系,江苏 南京 210013
Author(s):
WU Tong12WANG Zhenjiong2HUA Chun2ZHOU Feng2WANG Renlei3HUANG He1
1. College of Biotechnology and Pharmaceutical Engineering,Nanjing Tech University,Nanjing 211800,China; 2. School of Food Science,Nanjing Xiaozhuang College,Nanjing 211171,China; 3. Biology Department,Jiangsu Second Normal University,Nanjing 210013,China
关键词:
大肠杆菌MG1655 Na2SeO3 纳米硒 表征
Keywords:
Escherichia coli MG1655 sodium selenite nano-selenium characterization
分类号:
Q936
DOI:
10.3969/j.issn.1671-7627.2017.03.010
文献标志码:
A
摘要:
根据生物矿化策略,以不同质量浓度的Na2SeO3溶液作为硒源,利用大肠杆菌MG1655生物还原Na2SeO3制备纳米硒。研究Na2SeO3质量浓度对大肠杆菌生长曲线的影响,用氢化物原子荧光光谱法(HG-AFS)测定Na2SeO3的还原效率,进一步利用扫描电子显微镜(SEM)、X线衍射仪(XRD)和傅里叶红外光谱(FT-IR)等仪器对产物进行了相关表征。结果表明:大肠杆菌MG1655能将Na2SeO3大量矿化为粒径基本在100~250 nm左右的红色单质纳米硒颗粒,且对各质量浓度下的Na2SeO3的还原率均在50%以上。
Abstract:
The reduction with Escherichia coli MG1655 of sodium selenite was used to prepare nano-selenium with different mass concentrations of sodium selenite as selenium source artificially.The influence of sodium selenite mass concentration on the growth curve of Escherichia coli MG1655 was investigated.The reduction efficiency of sodium selenite was determined by hydride generation atomic fluorescence spectrometry(HG-AFS),and the structure of the product was characterized by scanning electron microscope(SEM),X-ray diffraction(XRD)and Fourier transform infrared spectroscopy(FT-IR).Results indicated that sodium selenite could be biomineralized in abundance by Escherichia coli MG1655 to nano-selenium particles in red simple substance with their diameters mainly 100~250 nm.Moreover,all the reduction rates of various mass concentrations of sodium selenite solution were over 50%.

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备注/Memo

备注/Memo:
收稿日期:2016-01-07
基金项目:国家自然科学基金(21376112); 江苏省自然科学基金(BK20131344)
作者简介:武童(1988—),女,江苏徐州人,硕士,主要研究方向为应用微生物; 华春(联系人),教授,E-mail:hc3501988@163.com.
引用本文:武童,汪振炯,华春,等.基于大肠杆菌MG1655载体矿化合成纳米硒的研究[J].南京工业大学学报(自然科学版),2017,39(3):52-57..
更新日期/Last Update: 2017-05-31