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[1]董丁,卢彦珍,唐美华,等.一株石油烃高效降解菌的筛选及降解性能研究[J].南京工业大学学报(自然科学版),2017,39(03):58-62.[doi:10.3969/j.issn.1671-7627.2017.03.011]
 DONG Ding,LU Yanzhen,TANG Meihua,et al.Screening of a high efficient bacterial strain for petroleum hydrocarbons degradation and its characteristics[J].Journal of NANJING TECH UNIVERSITY(NATURAL SCIENCE EDITION),2017,39(03):58-62.[doi:10.3969/j.issn.1671-7627.2017.03.011]
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一株石油烃高效降解菌的筛选及降解性能研究()
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《南京工业大学学报(自然科学版)》[ISSN:1671-7627/CN:32-1670/N]

卷:
39
期数:
2017年03期
页码:
58-62
栏目:
出版日期:
2017-05-20

文章信息/Info

Title:
Screening of a high efficient bacterial strain for petroleum hydrocarbons degradation and its characteristics
文章编号:
1671-7627(2017)03-0058-05
作者:
董丁卢彦珍唐美华陈国松
南京工业大学 化学与分子工程学院,江苏 南京 211800
Author(s):
DONG DingLU YanzhenTANG MeihuaCHEN Guosong
College of Chemistry and Molecular Engineering,Nanjing Tech University,Nanjing 211800,China
关键词:
石油烃 降解 枯草芽孢杆菌 含油泥砂
Keywords:
petroleum hydrocarbon degradation Bacillus subtilis oily sludge
分类号:
TE991.3
DOI:
10.3969/j.issn.1671-7627.2017.03.011
文献标志码:
A
摘要:
经原油-培养基驯化、LB平板分离、原油固体培养基平板嗜油斑分析,从含油泥砂中分离筛选出能够降解石油烃的菌株。经形态观察及16S rRNA基因序列分析鉴定,该菌株属于枯草芽孢杆菌(Bacillus subtilis),该菌株具有较高的石油烃降解能力。将10 mL该菌液用于处理5 g含油泥砂,7 d后石油烃降解率达33.52%,90 d后达90.61%。在定期补加营养液的条件下,60 d后石油烃降解率可达90.98%,大大缩短了处理时间。处理后泥砂含油率0.28%,低于国家农用土壤含油率0.3%的标准。该菌株在油田产出含油泥砂及被原油污染土壤的修复方面具有良好的应用前景。
Abstract:
Domestication by using base oil-inorganic salt culture medium,separation with Luria-Bertani(LB)plate and oil stain analysis,and a bacterial strain for petroleum hydrocarbons degradation was separated and screened from oily sludge.Morphologic observation and 16Sr RNA gene sequence identification indicated that it belonged to Bacillus subtilis with high ability to degrade petroleum hydrocarbons.Treating 5 g oily sludge by using 10 mL bacteria liquid,the petroleum hydrocarbons degradation rate achieved 33.52% and 90.61% separately after 7 d and 90 d.If nutrient solution was regularly supplied,the degradation rate would increased to 90.98% after 60 d with an obviously shortened time.The oil content of the sludge was 0.28%,lower than the limiting value 0.3% in the national standard of agricultural soil.This bacterial strain showed a good application prospect for the treatment of oily sludge and remediation of soil contaminated by crude oil.

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备注/Memo

备注/Memo:
收稿日期:2016-03-14
作者简介:董丁(1988—),男,湖北黄冈人,硕士,主要研究方向为被石油污染土壤的修复技术; 陈国松(联系人),教授,E-mail:gschen3303@sina.com.
引用本文:董丁,卢彦珍,唐美华,等.一株石油烃高效降解菌的筛选及降解性能研究[J].南京工业大学学报(自然科学版),2017,39(3):58-62..
更新日期/Last Update: 2017-05-31