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[1]郑晓霞,张诺,胡南.纸质文物上的“寄居者”[J].南京工业大学学报(自然科学版),2018,40(01):101-105.[doi:10.3969/j.issn.1671-7627.2018.01.016]
 ZHENG Xiaoxia,ZHANG Nuo,HU Nan."Dweller" on paper cultural relics[J].Journal of NANJING TECH UNIVERSITY(NATURAL SCIENCE EDITION),2018,40(01):101-105.[doi:10.3969/j.issn.1671-7627.2018.01.016]
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纸质文物上的“寄居者”()
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《南京工业大学学报(自然科学版)》[ISSN:1671-7627/CN:32-1670/N]

卷:
40
期数:
2018年01期
页码:
101-105
栏目:
出版日期:
2018-01-20

文章信息/Info

Title:
"Dweller" on paper cultural relics
文章编号:
1671-7627(2018)01-0101-05
作者:
郑晓霞1张诺2胡南1
1.南京工业大学 生物与制药工程学院,江苏 南京 211800; 2.南京博物院,江苏 南京 210016
Author(s):
ZHENG Xiaoxia1ZHANG Nuo2HU Nan1
1.College of Biological and Pharmaceutical Engineering, Nanjing Tech University,Nanjing 211800,China; 2.Nanjing Museum,Nanjing 210016,China
关键词:
纸质文物 菌种鉴定 酶活
Keywords:
paper cultural relics bacteria identification enzyme activity
分类号:
K878
DOI:
10.3969/j.issn.1671-7627.2018.01.016
文献标志码:
A
摘要:
为鉴定南京博物院馆藏书画覆背纸上的微生物种类,利用分子生物学方法,对污斑处进行分离及鉴定。为了验证实验获得的纯培养微生物是否具有降解和破坏纸张的能力,对分离微生物的纤维素酶酶活、木聚糖酶酶活及蛋白酶酶活进行了选择性测定。一共获得11株细菌和5株真菌,结果证实为9种细菌及2种真菌,它们主要属于芽孢杆菌属、链格孢属和木霉属。酶活测试显示,细菌中,泛菌属的纤维素酶酶活最高,短杆菌属的蛋白酶酶活最高,分别可达到1.76和6.57 U/mL。真菌的蛋白酶酶活普遍很低,木霉属的纤维素酶酶活和木聚糖酶酶活最高,分别是17.12和29.01 U/mL。实验结果有助于针对纸质文物的微生物侵蚀现象制定相关的保护措施。
Abstract:
In order to identify the microorganism species on the backside paper of painting and calligraphy(Nanjing Museum)with the utilizing of the molecular biological method,the microbe on stain were isolated and identified.With the purpose of detecting whether the purely cultured microorganisms had the ability to degrade and damage paper,the cellulase activity,xylanase activity and protease activity were measured.A total of 11 bacterial strains and 5 fungi were gathered,9 bacterial strains and 2 fungi were identified,and they belonged to Bacillus,Alternaria and Trichoderma mainly.According to the results of enzymatic determination,as to the bacteria,the cellulase activity of Pantoea was maximum,the protease activity of Brevibacterium was maximum of 1.76 U/mL and 6.57 U/mL respectively.The protease activity of fungi was quite low,the cellulase activity and xylanase activity of Trichoderma were maximum of 17.12 U/mL and 29.01 U/mL respectively.The results are useful to develop the protective techniques to against the microbial erosion on paper culture relics.

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备注/Memo

备注/Memo:
收稿日期:2016-12-28
作者简介:郑晓霞(1992—),女,浙江台州人,硕士,主要研究方向为基础微生物; 胡南(联系人),副教授,E-mail:hunan@njtech.edu.cn.
引用本文:郑晓霞,张诺,胡南.纸质文物上的“寄居者”[J].南京工业大学学报(自然科学版),2018,40(1):101-105..
更新日期/Last Update: 2018-01-18