|本期目录/Table of Contents|

[1]张楚阳,张珂嘉,刘琴.矢车菊素与矢车菊素-3-O-葡萄糖苷对H2O2诱导HepG-2细胞氧化应激保护作用的比较[J].南京工业大学学报(自然科学版),2018,40(03):31-37.[doi:10.3969/j.issn.1671-7627.2018.03.006]
 ZHANG Chuyang,ZHANG Kejia,LIU Qin.Comparison of protective effects of cyanidin and cyanidin-3-O-glucoside on oxidative stress induced by H2O2 in HepG-2 cell model[J].Journal of NANJING TECH UNIVERSITY(NATURAL SCIENCE EDITION),2018,40(03):31-37.[doi:10.3969/j.issn.1671-7627.2018.03.006]
点击复制

矢车菊素与矢车菊素-3-O-葡萄糖苷对H2O2诱导HepG-2细胞氧化应激保护作用的比较()
分享到:

《南京工业大学学报(自然科学版)》[ISSN:1671-7627/CN:32-1670/N]

卷:
40
期数:
2018年03期
页码:
31-37
栏目:
出版日期:
2018-05-20

文章信息/Info

Title:
Comparison of protective effects of cyanidin and cyanidin-3-O-glucoside on oxidative stress induced by H2O2 in HepG-2 cell model
文章编号:
1671-7627(2018)03-0031-07
作者:
张楚阳张珂嘉刘琴
南京财经大学 食品科学与工程学院 江苏省现代粮食流通与安全协同创新中心江苏高校粮油质量安全控制及深加工重点实验室,江苏 南京 210023
Author(s):
ZHANG ChuyangZHANG KejiaLIU Qin
Key Laboratory of Grains and Oils Quality Control and Processing,Collaborative Innovation Center for Modern Grain Circulation and Safety,College of Food Science and Engineering,Nanjing University of Finance and Economics,Nanjing 210023,China
关键词:
矢车菊素 矢车菊素-3-O-葡萄糖苷 H2O2 氧化应激模型
Keywords:
cyanidin cyanidin-3-O-glucoside hydrogen peroxide oxidative stress model
分类号:
TQ028.8
DOI:
10.3969/j.issn.1671-7627.2018.03.006
文献标志码:
A
摘要:
以人肝癌细胞HepG-2为模型,以细胞抗氧化水平为指标,比较研究一种花色苷元矢车菊素(Cy)和其对应的花色苷矢车菊素-3-O-葡萄糖苷(C3G)对H2O2诱导的细胞氧化应激的保护作用。采用噻唑蓝(MTT)法检测细胞的存活率,采用总抗氧化能力(T-AOC)和细胞中的超氧化物歧化酶(SOD)活力、谷胱甘肽(GSH)及丙二醛(MDA)的含量评价样品的抗氧化保护作用。结果表明:两种花青素在0~0.5 mmol/L范围内对HepG-2 细胞无明显的毒性; 与H2O2氧化应激模型组相比,在高(400 μmol/L)、中(200 μmol/L)、低(50 μmol/L)3种浓度下两种结构的花青素处理均可显著提高细胞总抗氧化能力、超氧化物歧化酶活力和还原型谷胱甘肽含量,降低细胞内丙二醛含量,抗氧化能力与花青素浓度呈正相关性; 而Cy的细胞抗氧化作用显著高于C3G。该研究结果为阐明花青素的构效关系提供了体外研究的新证据。
Abstract:
Protection effects of cyanidin(Cy)and its derivate cyanidin-3-O-glucoside(C3G)on cellular oxidative stress induced by H2O2 on HepG-2 cell line were compared.The cell viabilities were detected by thiazolyl blue tetrazolium bromide(MTT)assay and the antioxidant protection were evaluated by total antioxidant capacity(T-AOC),the activity of superoxide dismutase(SOD),concentration of glutathione(GSH)and malondialdehyde(MDA)in cell.Results showed that two anthocyanins had no significant toxic effects on cultured HepG-2 cells under the concentration range of 0-0.5 mmol/L.Compared with H2O2 control group,both anthocyanins could increase the levels of T-AOC and SOD,concentration of GSH,and decrease the concentration of MDA in HepG-2 cells at different concentration of 50,200 and 400 μmol/L.The antioxidant capacity positively correlated with the concentration of anthocyanins.The antioxidant effect of Cy was significantly higher than that of C3G.The study could provide a theoretical basis for further understanding the structure-activity relationship of anthocyanin in vivo.

参考文献/References:

[1] MARITIM A C,SANDERS R A,LII J B W.Diabetes,oxidative stress,and antioxidants:a review[J].Journal of biochemical and molecular toxicology,2010,17(1):24.
[2] NORDBERG J,ARNÉR E S.Reactive oxygen species,antioxidants,and the mammalian thioredoxin system[J].Free radical biology & medicine,2001,31(11):1287.
[3] FRANDSEN J R,NARAYANASAMY P.Neuroprotection through flavonoid:enhancement of the glyoxalase pathway[J].Redox biology,2017,14(C):465.
[4] NAEIMI A F,ALIZADEH M.Antioxidant properties of the flavonoid fisetin:an updated review of in vivo and in vitro studies[J].Trends in food science & technology,2017,70:34.
[5] 柯增光,许伟,邵荣,等.蓖麻碱的超声波辅助提取及其抗氧化性能[J].南京工业大学学报(自然科学版),2015,37(1):14.
[6] PUSPITA S,CHRISTOFORA H W,DONDIN S,et al.Colour properties,stability,and free radical scavenging activity of jambolan(Syzygium cumini)fruit anthocyanins in a beverage model system:natural and copigmentedanthocyanins[J].Food chemistry,2012,132(4):1908.
[7] CHANG L Z,ZHONG J C,XUE S B,et al.Structure-activity relationships of anthocyanidin glycosylation[J].Molecular diversity,2014,18(3):687.
[8] HUANG W Y,LIU Y M,WANG J,et al.Anti-inflammatory effect of the blueberry anthocyanins malvidin-3-glucoside and malvidin-3-galactoside in endothelial cells[J].Molecules,2014,19(8):12827.
[9] PILACZYNSKA-SZCZESNIAK L,SKARPANSKA-STEINBORN A,DESKUR E,et al.The influence of chokeberry juice supplementation on the reduction of oxidative stress resulting from an incremental rowing ergometer exercise[J].International journal of sport nutrition & exercise metabolism,2005,15(1):48.
[10] 程琤,刘超,贺炜,等.紫甘薯花青素的稳定性及抗氧化性研究[J].营养学报,2011,33(3):291.
[11] YAN F,CHEN Y,AZAT R,et al.Mulberry anthocyanin extract ameliorates oxidative damage in HepG2 cells and prolongs the lifespan of caenorhabditis elegans through MAPK and Nrf2 pathways[J].Oxidative medicine and cellular longevity,2017(1):1.
[12] CHEN Z,WANG C,PAN Y,et al.Hypoglycemic and hypolipidemic effects of anthocyanins extract from black soybean seed coat in high fat diet and streptozotocin-induced diabetic mice[J].Food & function,2017,doi:10.1039/c7fo00983f.
[13] SHAN Q,ZHENG Y,LU J,et al.Purple sweet potato color ameliorates kidney damage via inhibiting oxidative stress mediated NLRP3 inflammasome activation in high fat diet mice[J].Food & chemical toxicology,2014,69:339.
[14] CÁSEDAS G,LES F,GÓMEZ-SERRANILLOS M P,et al.Anthocyanin profile,antioxidant activity and enzyme inhibiting properties of blueberry and cranberry juices:a comparative study[J].Food & function,2017,8(11):4187.
[15] MANACH C,WILLIAMSON G,MORAND C,et al.Bioavailability and bioefficacy of polyphenols in humans:review of 97 bioavailability studies[J].The American journal of clinical nutrition,2005,81(1):230S.
[16] HIDALGO M,MARTIN-SANTAMARIA S,RECIO I,et al.Potential anti-inflammatory,anti-adhesive,anti/estrogenic,and angiotensin-converting enzyme inhibitory activities of anthocyanins and their gut metabolites[J].Genes & nutrition,2012,7(2):295.
[17] KALT W,MCDONALD J E,LIU Y,et al.Flavonoid metabolites in human urine during blueberry anthocyanin intake[J].Journal of agricultural & food chemistry,2017,65(8):1582.
[18] KALT W,LIU Y,MCDONALD J E,et al.Anthocyanin metabolites are abundant and persistent in human urine[J].Journal of agricultural & food chemistry,2014,62:3926.
[19] 冯所兰,周淅赟,牛文慧,等.黑米麸皮花青素脂质体的稳定性研究[J].食品工业科技,2015,36(22):115.
[20] KANETO H,KAWAMORI D,NAKATANI Y,et al.Oxidative stress and the JNK pathway as a potential therapeutic target for diabetes[J].Drug news & perspectives,2004,17(7):447.

备注/Memo

备注/Memo:
收稿日期:2018-01-07
基金项目:江苏省高校自然科学研究重大项目(11KJA550001); 江苏高校优势学科建设工程
作者简介:张楚阳(1993—),男,山东济宁人,硕士,主要研究方向为食品科学; 刘琴(联系人),教授,E-mail:liuqin31@njue.edu.cn.
引用本文:张楚阳,张珂嘉,刘琴.矢车菊素与矢车菊素-3-O-葡萄糖苷对H2O2诱导HepG-2细胞氧化应激保护作用的比较[J].南京工业大学学报(自然科学版),2018,40(3):31-37..
更新日期/Last Update: 2018-05-31