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[1]林军章,冯云,谭晓明,等.原油厌氧微生物降解特征分析[J].南京工业大学学报(自然科学版),2018,40(03):49-54.[doi:10.3969/j.issn.1671-7627.2018.03.009]
 LIN Junzhang,FENG Yun,TAN Xiaoming,et al.Characteristics of crude oil biodegradation by anaerobic microbial[J].Journal of NANJING TECH UNIVERSITY(NATURAL SCIENCE EDITION),2018,40(03):49-54.[doi:10.3969/j.issn.1671-7627.2018.03.009]
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原油厌氧微生物降解特征分析()
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《南京工业大学学报(自然科学版)》[ISSN:1671-7627/CN:32-1670/N]

卷:
40
期数:
2018年03期
页码:
49-54
栏目:
出版日期:
2018-05-20

文章信息/Info

Title:
Characteristics of crude oil biodegradation by anaerobic microbial
文章编号:
1671-7627(2018)03-0049-06
作者:
林军章1冯云1谭晓明1刘来燕23承磊23汪卫东1
1.中国石油化工集团公司 胜利油田分公司 石油工程技术研究院,山东 东营 257000; 2.农业部农村可再生能源开发利用重点实验室,四川 成都 610041; 3.农业部沼气科学研究所,四川 成都 610041
Author(s):
LIN Junzhang1FENG Yun1TAN Xiaoming1LIU Laiyan23CHENG Lei23WANG Weidong1
1.Petroleum Engineering Technology Research Institute,Shengli Oil Field Branch,Sinopec Group,Dongying 257000,China; 2.Key Laboratory of Rural Renewable Energy Development and Utilization,Ministry of Agriculture, Chengdu 610041,China; 3.Biogas Science Research Institute of Ministry of Agriculture,Chengdu 610041,China
关键词:
原油生物降解 厌氧降解 稠油形成 甲烷
Keywords:
crude oil biodegradation anaerobic microbial heavy oil formation methane
分类号:
TE357.9
DOI:
10.3969/j.issn.1671-7627.2018.03.009
文献标志码:
A
摘要:
为了研究原油厌氧微生物降解形成的原油组分和伴生气特征,在亨盖特厌氧微生物操作平台上,利用厌氧降解菌群对胜利油田两种稀油进行模拟降解研究。结果表明:稀油经过微生物厌氧降解具有稠化的趋势,伴随降解产生CH4和少量CO2,降解248 d每克原油能够产3.0 mmol CH4,补充矿物质营养后每克原油(滨76)产CH4达到6.4 mmol。不同原油降解产生的气体碳同位素存在显著差异,义141原油所产气体CH413C同位素比值偏差(δ(13C))分布为-4.636%~-4.527%,CO2δ(13C)分布为0.424%~0.850%; 而滨76原油降解所产气体CH4δ(13C)分布为-4.983%~-4.853%,CO2δ(13C)分布为0.254%~0.465%,碳同位素相对值差异性是由原油自身碳同位素组成差异性所导致。两种原油的饱和烃组分均被显著降解,最高由初始的72.77%下降到44.0%,但两种原油的胆甾烷等生物标志物在降解前后无显著变化,表明原油仍处于轻度生物降解阶段。室内模拟研究揭示的原油厌氧微生物降解过程的油气特征可为生物成因的稠油及伴生气的勘探开发提供重要理论指导。
Abstract:
To study the characteristics of crude oil biodegradation and transform to heavy oil,the oil came from Shengli Oil Field was selected to carried out biodegradation experiments on the Henget anaerobic microorganism operation platform.Results showed that the thin oil could be biodegraded and transformed to heavy oil.The degradation of 248 d per gram of crude oil could produce 3.0 mmol methane,even could be reached 6.4 mmol methane(B76)after supplying mineral nutrition.The isotope-ratio deviation of 13C(δ(13C))of methane coming from Y141 oil distributed from -4.636% to -4.527% and the δ(13C)of CO2 distributed from 0.424% to 0.850%,and the δ(13C)of methane coming from B76 oil distributed from -4.983% to -4.853% and the δ(13C)of CO2 distributed from 0.254% to 0.465%.Saturated hydrocarbon of crude oil was degraded greatly from 72.77% to 44.0%.There were no significant changes in two kinds of crude oil,such as cholesterol,alkane and other biomarkers,which indicated the oil had suffered little biodegradation.The characters of oil and gas in the process were important for exploration and development of heavy oil.

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备注/Memo

备注/Memo:
收稿日期:2017-11-27
基金项目:农业部农村可再生能源开发利用重点实验开放研究课题基金(2017003)
作者简介:林军章(1979—),男,辽宁大连人,高级工程师,博士,主要研究方向为石油微生物技术,E-mail:linjunzhang.slyt@sinopec.com.
引用本文:林军章,冯云,谭晓明,等.原油厌氧微生物降解特征分析[J].南京工业大学学报(自然科学版),2018,40(3):49-54..
更新日期/Last Update: 2018-05-31