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[1]梁颖,赵维,生弘杰,等.沙培体系下植物和生物质炭对胞外DNA残留的影响[J].南京工业大学学报(自然科学版),2019,41(05):632-637.[doi:10.3969/j.issn.1671-7627.2019.05.014]
 LIANG Ying,ZHAO Wei,SHENG Hongjie,et al.Effects of plant and maize straw biochar on DNA residues in sand culture[J].Journal of NANJING TECH UNIVERSITY(NATURAL SCIENCE EDITION),2019,41(05):632-637.[doi:10.3969/j.issn.1671-7627.2019.05.014]
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沙培体系下植物和生物质炭对胞外DNA残留的影响()
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《南京工业大学学报(自然科学版)》[ISSN:1671-7627/CN:32-1670/N]

卷:
41
期数:
2019年05期
页码:
632-637
栏目:
出版日期:
2019-10-22

文章信息/Info

Title:
Effects of plant and maize straw biochar on DNA residues in sand culture
文章编号:
1671-7627(2019)05-0632-06
作者:
梁颖12赵维1生弘杰2付玉豪2王心情1王芳2
1.南京工业大学 环境科学与工程学院,江苏 南京 211800; 2. 中国科学院 南京土壤研究所 土壤环境与污染修复重点实验室, 江苏 南京 210008
Author(s):
LIANG Ying12 ZHAO Wei1 SHENG Hongjie2 FU Yuhao2 WANG Xinqing1 WANG Fang2
1.School of Environmental Science and Engineering, Nanjing Tech University, Nanjing 211800, China; 2. Key Laboratory of Soil Environment and Pollution Remediation,Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008,China
关键词:
盆栽植物 生物质炭 DNA残留 抗性基因
Keywords:
potplants biochar DNA residual antibiotic resistance genes
分类号:
X53
DOI:
10.3969/j.issn.1671-7627.2019.05.014
文献标志码:
A
摘要:
为了研究植物和生物质炭对抗性DNA在土壤中残留变化的影响,采用沙培生菜盆栽实验,以小牛胸腺DNA为模拟抗性DNA,测定在栽培生菜和添加玉米秸秆炭处理中DNA的含量随时间的变化。结果表明:沙培期间,所有处理(对照、生菜栽培、添加玉米秸秆炭、生菜-玉米秸秆炭联合处理)中,DNA的含量均呈先快速下降,之后趋于平稳的趋势。相比对照,种植生菜使DNA在快速减少后小幅增加,仅添加生物质炭的处理在培养期间DNA含量均低于对照。植物可能通过根系影响微生物量增加DNA,玉米秸秆炭可能通过吸附作用减小DNA的含量,种植生菜或添加玉米秸秆炭减缓DNA降解速率的机制可能不同。该结果为抗性DNA和抗性基因在土壤-植物中迁移和传播阻控研究提供了采样时间的依据,有利于科学评价抗性基因迁移风险,但植物和玉米秸秆炭对DNA变化的影响和机制需进一步研究。
Abstract:
To study the residual changes of antibiotic resistance DNA affected by plants and maize straw biochar in soil, a sand culture pot experiment of lettuce was designed with calf thymus DNA used as a simulated resistance DNA. The content of simulated resistant DNA over time during plant cultivation and biochar treatment was determined. Results showed that DNA concentration in all the treatments(blank control, lettuce cultivation, maize straw biochar, and lettuce-biochar combined treatment)had a rapid decline at the early stage and then a slow decrease. Cultivation of lettuce or corn straw biochar addition could decrease the degradation rate of DNA. Regardless of adding biochar or not, compared to the control, growing lettuce treatment showed a small increase in DNA concentration after a rapid decrease period. The DNA content of the treatment with only adding maize straw biochar was lower than that of the control group during the culture period. The lettuce might increase concentrations of DNA via the microorganism in the rhizosphere. The maize straw biochar might decrease the contents of DNA and the degradation rate of DNA by adsorption of DNA via biochar micropores. The results provided a basis for sampling time for the migration, prevention and control of antibiotic resistant DNA and antibiotic resistant genes in soil-plant system, which was helpful in scientific evaluation of the risk of resistance gene migration. The effects and mechanism of maize straw biochar and plants on the changes of antibiotic resistant DNA concentration needed further study.

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备注/Memo

备注/Memo:
收稿日期:2019-07-10
基金项目:国家自然科学基金(21677149,41830642)
作者简介:梁颖(1995—),女,E-mail:liang121@njtech.edu.cn; 赵维(联系人),副教授,wzhao@njtech.edu.cn.
引用本文:梁颖,赵维,生弘杰,等.沙培体系下植物和生物质炭对胞外DNA残留的影响[J].南京工业大学学报(自然科学版),2019,41(5):632-637..
更新日期/Last Update: 2019-09-30