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[1]卢俊,韩静,金磊磊,等.NaCl胁迫对三叶半夏丛生芽生长的影响[J].南京工业大学学报(自然科学版),2020,42(02):257-261.[doi:10.3969/j.issn.1671-7627.2020.02.018]
 LU Jun,HAN Jing,JIN Leilei,et al.Effects of NaCl stress on cluster buds growth of Pinellia ternata[J].Journal of NANJING TECH UNIVERSITY(NATURAL SCIENCE EDITION),2020,42(02):257-261.[doi:10.3969/j.issn.1671-7627.2020.02.018]
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NaCl胁迫对三叶半夏丛生芽生长的影响()
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《南京工业大学学报(自然科学版)》[ISSN:1671-7627/CN:32-1670/N]

卷:
42
期数:
2020年02期
页码:
257-261
栏目:
出版日期:
2020-03-22

文章信息/Info

Title:
Effects of NaCl stress on cluster buds growth of Pinellia ternata
文章编号:
1671-7627(2020)02-0257-05
作者:
卢俊1韩静1金磊磊1贾启2陈集双12
1.南京工业大学 生物与制药工程学院,江苏 南京 211800; 2.遵义医科大学 生物资源健康利用研究中心,贵州 遵义 563000
Author(s):
LU Jun1 HAN Jing1 JIN Leilei1 JIA Qi2 CHEN Jishuang12
1. College of Biotechnology and Pharmaceutical Engineering,Nanjing Tech University,Nanjing 211800,China; 2. Bioresource Institute of Healthy Utilization, Zunyi Medical University, Zunyi 563000, China
关键词:
三叶半夏 NaCl胁迫 丛生芽生长 离子毒害 丛生芽诱导
Keywords:
Pinellia ternata NaCl stress cluster buds growth ion toxic cluster buds induction
分类号:
Q813.1
DOI:
10.3969/j.issn.1671-7627.2020.02.018
文献标志码:
A
摘要:
为了获得大量健壮的三叶半夏组培苗,考察NaCl胁迫对三叶半夏丛生芽生长的影响。在三叶半夏丛生芽的增殖阶段,分别加入0、1、2、3和4 g/L NaCl,培养30 d后,测定丛生芽的外观形态和增殖状况。结果表明:在丛生芽增殖阶段,随着NaCl质量浓度的增加,T2与T2+丛生芽的增殖系数、叶柄长度和叶柄直径先增后降,丛生芽的干物率与NaCl质量浓度呈正相关; NaCl质量浓度为1和2 g/L时,T2与T2+丛生芽的生长优于对照组; NaCl质量浓度为2 g/L时,T2与T2+丛生芽的增殖系数分别比对照组提高了3.14%和4.86%,T2与T2+丛生芽的叶柄长度分别比对照组提高了9.23%和9.05%,T2与T2+丛生芽的叶柄直径分别比对照组提高了8.65%和7.62%; NaCl质量浓度为3和4 g/L时,丛生芽的生长受到明显抑制。
Abstract:
To obtain a large number of robust tissue culture seedlings, the effects of NaCl stress on cluster buds growth of Pinellia ternata(P. ternata)were investigated. In the proliferation stage of cluster buds of P. ternata, 0, 1, 2, 3 and 4 g/L NaCl were added to the cluster buds culture medium. After 30 d, the plant morphology and proliferation of cluster buds were determined. Results revealed that the proliferation coefficient, petiole length and petiole diameter of T2 and T2+ cluster buds increased at first and then decreased, and the dry matter was positively correlated with the concentration of NaCl. When the mass concentration of NaCl was 1 and 2 g/L, the growth of T2 and T2+ cluster buds were better than that of the control group. At 2 g/L NaCl, the proliferation coefficient of T2 and T2+ cluster buds increased by 3.14% and 4.86% respectively, the petiole length of T2 and T2+ cluster buds increased 9.23% and 9.05% respectively, and the petiole diameter of T2 and T2+ cluster buds increased 8.65% and 7.62% respectively as compared with the control group. When the mass concentration of NaCl reached 3 and 4 g/L, the growth of cluster buds was significantly inhibited.

参考文献/References:

[1] 曹礼.旱稻对盐胁迫的生理响应[D].兰州:兰州大学,2006.
[2] 刘祖祺,张石城.植物抗性生理学[M].北京:中国农业出版社,1994.
[3] TAVAKKOLI E,FATEHI F,COVENTRY S,et al.Additive effects of Na+and Cl- ions on barley growth under salinity stress[J].Journal of Experimental Botany,2011,62(6):2189.
[4] 列志旸,薛立.盐胁迫对树木生长影响研究综述[J].世界林业研究,2017,30(3):30.
[5] 杨秀艳,张华新,张丽,等.NaCl胁迫对唐古特白刺幼苗生长及离子吸收、运输与分配的影响[J].林业科学,2013,49(9):165.
[6] 孙仁国,赵桂琴,胡凯军,等.盐胁迫对燕麦地上干物质积累及灌浆期光合特性的影响[J].中国草地学报,2010,32(5):15.
[7] 申玉香,王爱民,李洪山,等.盐分胁迫对宁盐1号小麦群体质量、产量及蛋白质含量的影响[J].安徽农业科学,2008,36(34):14866.
[8] 周莹,赵永娟,黄丽瑾,等.荆芥幼苗对盐胁迫的生理响应[J].核农学报,2018,33(1):166.
[9] 陈炳东,黄高宝,陈玉梁,等.盐胁迫对油葵根系活力和幼苗生长的影响[J].中国油料作物学报,2008,30(3):327.
[10] 王吉明,马双武.NaCl胁迫对西瓜种子发芽的影响[J].北方园艺,2007(3):20.
[11] 孙敏红,邓云,相琼.NaCl胁迫对不同西瓜品种种子发芽及其幼苗生长特性的影响[J].北方园艺,2011(13):24.
[12] 杨姝,杜桂娟.盐胁迫下沙打旺种子的萌发特性[J].现代畜牧兽医,2011(1):50.
[13] 王荣华,石雷,汤庚国,等.盐胁迫下蒙古冰草幼苗生长和离子含量的变化[J].植物研究,2004(3):326.
[14] 陈阳春.盐胁迫对半夏组培苗生长及生理的影响[D].南京:南京工业大学,2014.
[15] 国家药典委员会.中华人民共和国药典[M].2005年版.北京:化学工业出版社,2005.
[16] TOMODA M,GONDA R,OHARA N,et al.A glucan having reticuloendothelial system-potentiating and anti-complementary activities from the tuber of Pinellia ternata[J].Biological & Pharmaceutical Bulletin,1994,17(6):859.
[17] 陈集双,欧阳平凯,贾明良.半夏资源生物资源与细胞工程[M].北京:科学出版社,2012.
[18] 金银兵,杨宗其,陈集双.三叶半夏组培快繁与优良单株的选育[J].中国生物工程杂志,2008,28(6):48.
[19] 贾明良.新品系半夏T2+的特性及规模化扩繁技术研究[D].南京:南京工业大学,2013.
[20] 赵可夫.NaCl抑制棉花幼苗生长的机理:盐离子效应[J].植物生理学报,1989,15(2):173.
[21] 朱燕芳,王延秀,陈佰鸿,等.四种苹果砧木幼苗对NaCl胁迫的生理响应、根系生长及耐盐性评价[J].甘肃农业大学学报,2017,52(2):48.
[22] 周志林,唐君,曹清河,等.NaCl胁迫对甘薯植株体内K+、Na+和Cl-含量及生长的影响[J].中国农业科技导报,2017,19(4):17.
[23] 王雪青,张俊文,魏建华,等.盐胁迫下野大麦耐盐生理机制初探[J].华北农学报,2007,22(1):17.
[24] 赵可夫,范海.盐生植物及其对盐渍生境的适应研究[M].北京:科学出版社,2005.
[25] 韩志平,郭世荣,冯吉庆,等.盐胁迫对西瓜幼苗生长、叶片光合色素和脯氨酸含量的影响[J].南京农业大学学报,2008,31(2):32.

备注/Memo

备注/Memo:
收稿日期:2019-06-26
基金项目:国家自然科学基金(31560079)
作者简介:卢俊(1994—),男,E-mail:1554235846@qq.com; 陈集双(联系人),教授,E-mail:biochenjs@njtech.edu.cn.
引用格式:卢俊,韩静,金磊磊,等.NaCl胁迫对三叶半夏丛生芽生长的影响[J].南京工业大学学报(自然科学版),2020,42(2):257-261.
LU Jun, HAN Jing, JIN Leilei, et al. Effects of NaCl stress on cluster buds growth of Pinellia ternata[J].Journal of Nanjing Tech University(Natural Science Edition),2020,42(2):257-261..
更新日期/Last Update: 2020-03-20